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Notwithstanding the variety of strategies that have been released to beat artefacts connected with info compositionality, they may have not been adopted with the human microbiome study. Of note, none of the medical trials posted throughout the earlier yr (apart from the ones particularly addressing the compositionality challenge [five, six]) used quantitative microbiome profiling. Here, we present how quantitative PCR (qPCR)-based mostly bacterial enumeration could be built-in to NGS pipelines to provide a possible method of estimate absolute abundances from NGS details, and hence endorse the usage of quantitative microbiome profiling in the sphere of human microbiome.
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The sequencing reads assigned to diverse taxa in Every sample had been divided by the total quantity of reads for that sample to obtain relative abundances in the taxa in Just about every sample. The relative abundances attained based upon the sequencing reads were translated into complete abundances by multiplying check here the relative abundance of each taxon by the entire bacterial abundance in the sample.
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